Supporting Online Material for Functional Proteomics Identify Cornichon Proteins as Auxiliary Subunits of AMPA Receptors

نویسندگان

  • Jochen Schwenk
  • Nadine Harmel
  • Gerd Zolles
  • Wolfgang Bildl
  • Akos Kulik
  • Bernd Heimrich
  • Osamu Chisaka
  • Peter Jonas
  • Uwe Schulte
  • Bernd Fakler
  • Nikolaj Klöcker
چکیده

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منابع مشابه

Functional proteomics identify cornichon proteins as auxiliary subunits of AMPA receptors.

Glutamate receptors of the AMPA-subtype (AMPARs), together with the transmembrane AMPAR regulatory proteins (TARPs), mediate fast excitatory synaptic transmission in the mammalian brain. Here, we show by proteomic analysis that the majority of AMPARs in the rat brain are coassembled with two members of the cornichon family of transmembrane proteins, rather than with the TARPs. Coassembly with c...

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Jochen Schwenk , as Auxiliary Subunits of AMPA Receptors Functional Proteomics Identify Cornichon

www.sciencemag.org (this information is current as of March 12, 2009 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/323/5919/1313 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/323/5919/1313/DC1 can be foun...

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Auxiliary Subunits: Shepherding AMPA Receptors to the Plasma Membrane

Ionotropic glutamate receptors (iGluRs) are tetrameric ligand-gated cation channels that mediate excitatory signal transmission in the central nervous system (CNS) of vertebrates. The members of the iGluR subfamily of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors (AMPARs) mediate most of the fast excitatory signal transmission, and their abundance in the postsynaptic me...

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AMPA Receptors Commandeer an Ancient Cargo Exporter for Use as an Auxiliary Subunit for Signaling

Fast excitatory neurotransmission in the mammalian central nervous system is mainly mediated by ionotropic glutamate receptors of the AMPA subtype (AMPARs). AMPARs are protein complexes of the pore-lining α-subunits GluA1-4 and auxiliary β-subunits modulating their trafficking and gating. By a proteomic approach, two homologues of the cargo exporter cornichon, CNIH-2 and CNIH-3, have recently b...

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Cornichon Proteins Determine the Subunit Composition of Synaptic AMPA Receptors

Cornichon-2 and cornichon-3 (CNIH-2/-3) are AMPA receptor (AMPAR) binding proteins that promote receptor trafficking and markedly slow AMPAR deactivation in heterologous cells, but their role in neurons is unclear. Using CNIH-2 and CNIH-3 conditional knockout mice, we find a profound reduction of AMPAR synaptic transmission in the hippocampus. This deficit is due to the selective loss of surfac...

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تاریخ انتشار 2009